H2B K15ac Antibody

• Catalog Number: orb1259420
• Category: Antibodies
• Description: H2B K15ac Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H2B K15ac
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (orb1259420) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.