H2AX Antibody

• Catalog Number: orb1563813
• Category: Antibodies
• Description: H2AX Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: R02-3C8
• Tested applications: ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A synthetic peptide of human Histone H2A.X
• Concentration: 0.3 mg/ml
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Target: H2AX
• UniProt ID: P16104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry analysis of Histone H2A.X (green) in Hela using Histone H2A.X antibody, and DAPI (blue).

Western blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.