H2AX Antibody

• Catalog Number: orb1563163
• Category: Antibodies
• Description: H2AX Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2A9-B5
• Tested applications: ICC, WB
• Reactivity: Human, Mouse
• Isotype: IgG2a
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
• Concentration: 6.67 mg/ml
• Dilution range: WB: 1/500-1/1000 IF: 1/50-1/200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Target: H2AX
• UniProt ID: P16104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (2A9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) (2A9) antibody.

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (2A9) in A549 (upper, untreated or Hydroxyureatreated) and Hela (lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.

Western blot analysis of Phospho-Histone H2A.X (Ser139) (2A9) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) (2A9) antibody (upper) or betaActin antibody (lower).