H2AFZ (Ab-4) Antibody

• Catalog Number: orb416638
• Category: Antibodies
• Description: H2AFZ (Ab-4) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Peptide sequence around site of Lys (4) derived from Human Histone H2A.Z
• Dilution range: WB:1:500-5000, IHC-P:1:20-200, IF:1:50-200, IP:1:200-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H2AFZ
• UniProt ID: P0C0S5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: Anti-Histone H2A.Z antibody, anti- H2A/z antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H2AFZ (Ab-4) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR

Western blot aanalysis of K562 whole cell lysate, Mouse spleen tissueusing H2AFZ (Ab-4) antibody

Immunoprecipitation analysis of K562 whole cell lysate using H2AFZ (Ab-4) antibody

Immunofluorescence staining of Hela cells using H2AFZ (Ab-4) antibody

Immunohistochemistry of paraffin-embedded human prostate tissue using H2AFZ (Ab-4) antibody

Immunohistochemistry of paraffin-embedded human brain tissue using H2AFZ (Ab-4) antibody