H2AFX (phospho-S139) antibody

• Catalog Number: orb318857
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2A.X (Phospho-S139)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2A.X protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100, FACS: 1:100-1:200M
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: H2AFX
• Entrez: 15270, 3014
• UniProt ID: P27661, P16104
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti H2AX antibody, anti Histone H2AX antibody, an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2A.X (Phospho-S139) expression in HEK293T (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 20 kD)

Immunohistochemical analysis of Histone H2A.X (Phospho-S139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H2A.X (Phospho-S139) staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).