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Catalog Number | orb1253452 |
---|---|
Category | Antibodies |
Description | H2AFX Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | Histone H2A.X (Phospho-Ser139) antibody was raised against a peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X. |
Concentration | 1 mg/mL |
Dilution range | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 15 kDa |
Target | H2AFX |
UniProt ID | P16104 |
NCBI | NP_002096.1 |
Storage | Store antibody at -20°C for up to one year. |
Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Alternative names | H2AX, H2A.X, H2A/X, HNF3B, TCF3B, H2AFX, H2a/x, HI Read more... |
Note | For research use only |
Application notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysed extracts from HT29 cells untreated (Lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139).
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X (Phospho-Ser139).
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Human, Mouse | |
Monoclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Human, Mouse | |
Monoclonal | |
Unconjugated |
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