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Catalog Number | orb1252534 |
---|---|
Category | Antibodies |
Description | H1F0 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1415-1 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG2a, kappa |
Immunogen | Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | H1F0 |
UniProt ID | P07305 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | H1F0, H10, H1FV, MGC5241 Read more... |
Note | For research use only |
Application notes | Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1).
FFPE human pancreas tested with Histone H1 antibody (1415-1).
FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1).
FFPE human angiosarcoma tested with Histone H1 antibody (1415-1).
Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ChIP, ELISA, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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