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Catalog Number | orb1263785 |
---|---|
Category | Antibodies |
Description | GZMB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the C-terminal region of human GZMB. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 28 kDa |
Target | GZMB |
UniProt ID | P10144 |
NCBI | P10144 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of GZMB Antibody in HL-60 cell line lysates (35 ug/lane)
GZMB Antibody IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GZMB using rabbit polyclonal GZMB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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