GZMB Antibody (C-term)

• Catalog Number: orb1929973
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19353
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 27716 Da
• Target: This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the C-terminal region of human GZMB.
• UniProt ID: P10144
• NCBI: NP_004122.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GZMB Antibody (C-term) in HL-60 cell line lysates (35 ug/lane). GZMB (arrow) was detected using the purified Pab.

Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.