Guinea Pig IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347099
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (Peroxidase)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Guinea pig
• Isotype: IgG
• Immunogen: Guinea Pig IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Donkey Anti-Guinea Pig IgG Peroxidase Conjugation,
• Note: For research use only
• Application notes: Anti-Guinea Pig IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Donkey Anti-Guinea Pig IgG mx10 Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG. The working dilution is 1:147000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using orb348651 substrate.