Guinea Pig IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

• Catalog Number: orb347096
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (TRITC)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF, WB
• Reactivity: Guinea pig
• Isotype: IgG
• Immunogen: Guinea Pig IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey Anti-Guinea Pig IgG Rhodamine Conjugation,
• Note: For research use only
• Application notes: Anti-Guinea Pig IgG Rhodamine Antibody has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western Blot of Donkey anti-Guinea Pig IgG Pre-Absorbed Rhodamine Conjugated Secondary Antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine donkey secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.