Guinea Pig IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347063
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Guinea pig
• Isotype: IgG
• Immunogen: Anti-Guinea Pig IgG whole molecule was produced by repeated immunization with Guinea Pig IgG whole molecule in goat.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:40,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat Anti-Guinea Pig IgG Antibody peroxidase Conju
• Note: For research use only
• Application notes: Anti-Guinea Pig IgG Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. It is also suitable in IHC. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified ELISA of Goat Anti-Guinea Pig IgG Antibody Peroxidase Conjugated tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (red line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.