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Catalog Number | orb346134 |
---|---|
Category | Proteins |
Description | Guinea Pig IgG Antibody |
Tested applications | ELISA, IHC, SDS-PAGE, WB |
Isotype | IgG |
Concentration | 10 mg/ml |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | Guinea Pig IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Guinea Pig IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig IgG and anti-Guinea Pig Serum. |
Conjugation | Unconjugated |
Source | Guinea Pig |
Biological Origin | Guinea Pig |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Guinea Pig IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | Guinea Pig Immunoglobulin G, GP IgG Read more... |
Note | For research use only |
Application notes | Guinea Pig IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS-Page of Guinea Pig IgG whole molecule. Lane 1: Guinea Pig IgG – Non-reduced. Lane 2: Guinea Pig IgG – Reduced. Load: 1.0 µg per lane. Predicted/Observed size: 25 & 55 kDa – Reduced, 160 kDa – Non-reduced for IgG whole molecule. Other band(s): None.
DOT, FC, FLISA, IF | |
Rabbit | |
Guinea pig | |
Polyclonal | |
TRITC |
ELISA, FC, IF, IH | |
Guinea pig | |
Rabbit | |
Polyclonal | |
FITC |
ELISA, IH, WB | |
Guinea pig | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IH, WB | |
Guinea pig | |
Rabbit | |
Polyclonal | |
Biotin |
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