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Catalog Number | orb344490 |
---|---|
Category | Antibodies |
Description | GSTO1 antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 14A9.F6 |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | This Protein A purified antibody was prepared by repeated immunizations in mice with O1 Protein |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:50,000 - 1:200,000, IHC: User Optimized, IP: User Optimized, WB: 1:500 - 1:1000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above. Reacts specifically with O1 protein. Cross reactivity from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P78417 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-GSTO1 antibody, mouse anti-GST-O1 antib Read more... |
Note | For research use only |
Application notes | Anti-GSTO1 antibody has been tested by ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Mouse anti-GSTO1 Monoclonal Antibody. Lane 1: Non-reduced Mouse anti-GSTO1 Monoclonal Antibody. Lane M: 3 µL OPAL Pre-stained Marker. Lane 2: Reduced Mouse anti-GSTO1 Monoclonal Antibody. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.
Western Blot of Mouse anti-GSTO1 Monoclonal Antibody. Lane 1: Recombinant GSTO1 protein. Lane 2: GST (p/n orb345956). Load: 50 ng per lane. Primary antibody: Mouse anti-GSTO1 Monoclonal Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP Mouse Secondary Antibody (orb347506) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTO1.
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