GST3/GST pi/GSTP1 Antibody

• Catalog Number: orb215938
• Category: Antibodies
• Description: GST3/GST pi/GSTP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23356 MW
• UniProt ID: P09211
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutathione S-transferase P;2.5.1.18;GST class-pi;
• Note: For research use only
• Application notes: WB: The detection limit for GST3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-GST3/GST pi antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GST3/GST pi using anti-GST3/GST pi antibody.Lane 1:human HeLa cell;2:human 293T cell;3:human Jurkat cell;4:mouse liver tissue.

IF analysis of GST3/GST pi using anti-GST3/GST pi antibody. GST3/GST pi was detected in immunocytochemical section of SH-SY5Y cells.

IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody. GST3/GST pi was detected in a paraffin-embedded section of rat lung tissue.

IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody. GST3/GST pi was detected in a paraffin-embedded section of human lung cancer tissue.