GST antibody (Peroxidase)

• Catalog Number: orb345850
• Category: Antibodies
• Description: GST antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Isotype: IgG
• Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Conjugation: HRP
• Storage: Store GST antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-GST antibody peroxidase conjugation, H
• Note: For research use only
• Application notes: Anti-GST Peroxidase conjugated Antibody has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western Blot showing detection of recombinant GST protein (0.25 µg) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 µM nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, unconjugated primary antibody (orb345365) was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.