GST antibody (Peroxidase)

• Catalog Number: orb345311
• Category: Antibodies
• Description: GST antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Isotype: IgG
• Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-GST HRP Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Conjugation: HRP
• Storage: Store anti-GST at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-GST antibody HRP conjugation, peroxidase
• Note: For research use only
• Application notes: Anti-GST peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using peroxidase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western Blot Results of Goat Anti-GST Antibody Peroxidase Conjugated. Lane 1: rec GST (p/n orb345956)/HeLa WCL (p/n orb348668) [0.03/10 µg]. Lane 2: HeLa Whole Cell Lysate (p/n orb348668) [10 µg]. Lane 3: 12 Epitope GST Lysate (p/n orb535115) [0.5 µg]. Lane 4: Opal Prestained 11-245 kDa Molecular Weight Marker. Antibody: Goat Anti-GST Peroxidase Conjugated Antibody at 1.0 µg overnight at 4°C. BlockOut blocking buffer (p/n orb348644) for 1 hr at RT. Predicted MW: 25 kDa. Observed MW: 25 kDa in lane 1. 48 kDa in lane 4 for 12 Epitope GST Lysate. Exposure: 0.03 seconds.