GRP94 Antibody / HSP90B1

• Catalog Number: orb639975
• Category: Antibodies
• Description: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HSP90B1/3168R
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant full-length human protein was used as the immunogen for this recombinant GRP94 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant GRP94 antibody is available for research use only.
• UniProt ID: P14625
• Storage: Store the recombinant GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human HepG2 cells with recombinant GRP94 antibody (clone HSP90B1/3168R); Red=isotype control, Blue= recombinant GRP94 antibody.

IHC staining of FFPE breast carcinoma with recombinant GRP94 antibody (clone HSP90B1/3168R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE breast carcinoma with recombinant GRP94 antibody (clone HSP90B1/3168R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant GRP94 antibody (clone HSP90B1/3168R) as confirmation of integrity and purity.