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Catalog Number | orb315148 |
---|---|
Category | Antibodies |
Description | GRP78 BiP/HSPA5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP (592-624aa ETMEKAVEEKIEWLESHQDADIEDFKAKKKELE), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 72333 MW |
UniProt ID | P11021 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | 78 kDa glucose-regulated protein;GRP-78;Endoplasmi Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of GRP78 using anti-GRP78 antibody.Lane 1:HELA Cell.
IHC analysis of GRP78 using anti-GRP78 antibody. GRP78 was detected in a paraffin-embedded section of mouse testis tissue.
IHC analysis of GRP78 using anti-GRP78 antibody. GRP78 was detected in a paraffin-embedded section of rat testis tissue.
IHC analysis of GRP78 using anti-GRP78 antibody. GRP78 was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of GRP78 using anti-GRP78 antibody. GRP78 was detected in a paraffin-embedded section of human lung cancer tissue.
ICC, IHC, WB | |
Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Bovine, Gallus, Mouse, Rat | |
Human, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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