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Catalog Number | orb1822404 |
---|---|
Category | Proteins |
Description | Active Human Recombinant GRP78 (Bip) Protein |
Tested applications | ATPase Activity Assay, ELISA, FA, SDS-PAGE, WB |
Reactivity | Human |
Tag | His tag |
Concentration | Lot/batch specific. See included datasheet. |
Purity | > 90% |
MW | ~78 kDa |
Target | GRP78 (Bip) |
UniProt ID | P11021 |
Protein Sequence | MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL |
Source | Recombinant |
Expression System | E. coli |
Biological Activity | ATPase active |
Storage | -20°C |
Buffer/Preservatives | 50mM Tris/HCl, pH 7.5, 150mM NaCl, 10% glycerol |
Alternative names | BIP Protein, Grp78 Protein, HSPA5 Protein, MIF2 Pr Read more... |
Note | For research use only |
Application notes | This product has been certified > 90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay. |
Expiration Date | 6 months from date of receipt. |
ELISA, ICC, IF, IHC-P, WB | |
Gallus | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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