GRP78 / BiP Antibody

• Catalog Number: orb1261886
• Category: Antibodies
• Description: GRP78 / BiP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 78kDa
• Target: HSPA5
• UniProt ID: P11021
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HSPA5 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.