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GRP78 / BiP Antibody
Catalog Number: orb1261265
| Catalog Number | orb1261265 |
|---|---|
| Category | Antibodies |
| Description | GRP78 / BiP Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1). |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 78kDa |
| Target | HSPA5 |
| UniProt ID | P11021 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HSPA5 antibody (orb1261265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry of paraffin-embedded rat testis using GRP78/BiP antibody (orb1261265) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GRP78/BiP antibody (orb1261265) at dilution of 1:100 (40x lens).
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HSPA5/GRP78/BiP Antibody [orb1535699]
ICC, IF, IHC, IHC-P, IP, WB
Human, Monkey, Mouse
Rabbit
Polyclonal
Unconjugated
50 μl - Item 1 of 1
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GRP78 (Bip) Antibody: APC [orb151963]
ICC, IF, IHC
Canine, Drosophila, Hamster, Human, Mouse, Rat
Rabbit
Polyclonal
APC
100 μg
