GRP78 Antibody

• Catalog Number: orb67383
• Category: Antibodies
• Description: Mouse Anti-Human GRP78 Monoclonal IgG2b
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1H11-1H7
• Tested applications: ICC, IF, WB
• Reactivity: Bovine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat
• Isotype: IgG2b
• Immunogen: His-tagged human GRP78
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Conjugation: Unconjugated
• MW: 78kDa
• Target: GRP78
• Entrez: 14828
• UniProt ID: P20029
• NCBI: NP_001156906.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: 78 kDa glucose regulated protein Antibody, 78 kDa
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-195 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western Blot analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody. Secondary Antibody: Goat anti-mouse IgG: HRP at 1:10000.