GRP78 Antibody: Biotin

• Catalog Number: orb181919
• Category: Antibodies
• Description: Mouse monoclonal antibody against GRP78, conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C5-1A4
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1 Kappa
• Immunogen: Full-length recombinant rat GRP78
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 78kDa
• Target: GRP78
• Entrez: 25617
• UniProt ID: P06761
• NCBI: NP_037215.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: 78 kDa glucose regulated protein antibody, 78 kDa
• Note: For research use only
• Application notes: 1 µg/ml of SMC-210 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody.