GRP78 Antibody: Biotin

• Catalog Number: orb148025
• Category: Antibodies
• Description: Mouse monoclonal to GRP78 (Biotin). GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the prope..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6H4-2G7
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Bovine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat
• Isotype: IgG1
• Immunogen: His-tagged human GRP78
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 78kDa
• Target: GRP78
• Entrez: 14828
• UniProt ID: P20029
• NCBI: NP_001156906.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: 78 kDa glucose regulated protein antibody, 78 kDa
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-196 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4-2G7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Western Blot analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.