Grin2b Antibody

• Catalog Number: orb1273518
• Category: Antibodies
• Description: Grin2b Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: NMDA NR2B (Tyr1472) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyr1472 of rat NMDA NR2B.
• Concentration: batch dependent
• Dilution range: The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 180
• Target: Grin2b
• UniProt ID: Q00960
• NCBI: Q00960
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472.