You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1273525 |
---|---|
Category | Antibodies |
Description | Gria1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Immunogen | GluR1 (Ser831) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser831 of rat GluR1. |
Concentration | batch dependent |
Dilution range | Immunolabeling of the GluR1 protein in Western blots of rat brain is blocked by the Ser831 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 100 |
Target | Gria1 |
UniProt ID | P19490 |
NCBI | P19490 |
Storage | For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
Note | For research use only |
Application notes | Immunolabeling of the GluR1 protein in Western blots of rat brain is blocked by the Ser831 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. |
Expiration Date | 12 months from date of receipt. |
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser831.
IHC, WB | |
Bovine, Canine, Equine, Monkey, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating