Gria1 Antibody

• Catalog Number: orb1273524
• Category: Antibodies
• Description: Gria1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1.
• Concentration: batch dependent
• Dilution range: Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100
• Target: Gria1
• UniProt ID: P19490
• NCBI: P19490
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845.