GRB2 Antibody

• Catalog Number: orb1247966
• Category: Antibodies
• Description: GRB2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-PRNYVTPVNRNV
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GRB2
• UniProt ID: P62993
• NCBI: NP_987102.1, NP_002077.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRB2, growth factor receptor-bound protein 2, ASH,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1247966 (0.05 ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35 ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247966 (0.05 ug/ml) staining of Human Thymus (A) and MOLT4 (B) lysates (35 ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247966 (0.03 ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247966 (1 ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.