GPX4/MCSP Antibody

• Catalog Number: orb1538019
• Category: Antibodies
• Description: GPX4/MCSP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human, Monkey
• Immunogen: Peptide with sequence C-EEPLVIEKDLPHY, from the C-Terminus of protein sequence according to NP_002076.2NP_001034937.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
• Conjugation: Unconjugated
• Target: GPX4 / MCSP
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydropero
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3246 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3246 was determined to be 2.5 ug/ml.
• Expiration Date: 12 months from date of receipt.

Antibody (0.1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.