GPR65 antibody

• Catalog Number: orb101581
• Category: Antibodies
• Description: GPR65 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GPR65 (51-120/337 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: GPR65
• UniProt ID: Q8IYL9
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: GPCR GPR65; Dig1; G protein-coupled receptor 65; G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin embedded human colon cancer tissue (dilution at:1:200) using GPR65 antibody

IHC-P of human colon cancer (Dilution at:1:200) using GPR65 antibody

Paraffin-embedded rat lung tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). GPR65 antibody at 1200 dilution with overnight incubation at 4 degrees

Paraffin-embedded mouse colon tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). GPR65 antibody at 1200 dilution with overnight incubation at 4 degrees

Paraffin-embedded human colon carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). GPR65 antibody at 1200 dilution with overnight incubation at 4 degrees

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-GPR65 (orb101581) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 44 kD