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Catalog Number | orb213930 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to GPC5 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Primate, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glypican 5. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1-1000, IP: 1-10-1-100 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | GPC5 |
Entrez | 103978, 2262 |
UniProt ID | Q8CAL5, P78333 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti Glypican-5 antibody Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Glypican 5 expression in Hela (A), mouse brain (B), mouse lung (C), mouse liver (D), rat brain (E), rat lung (F) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 70 kD)
Immunofluorescent analysis of Glypican 5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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