GPAM Antibody

• Catalog Number: orb1239417
• Category: Antibodies
• Description: GPAM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1.The immunogen is located within amino acids 730 - 780 of GPAT1.
• Concentration: 1 mg/mL
• Dilution range: GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 87, 91 kDa Observed: 92 kDa
• Target: GPAM
• UniProt ID: Q9HCL2
• NCBI: NP_065969
• Storage: GPAT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-
• Note: For research use only
• Application notes: GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 µg/mL.

Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 µg/mL.

Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 µg/ml.

Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20 µg/mL. Green: GPAT1 Antibody (orb1239417) Blue: DAPI staining.