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Catalog Number | orb1477984 |
---|---|
Category | Proteins |
Description | Recombinant Gossypium hirsutum protein BRASSINAZOLE-RESISTANT 1-like(LOC107910168)(H295N) |
Tag | N-terminal 6xHis-SUMO-tagged and C-terminal 6xHis-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 85% as determined by SDS-PAGE. |
MW | 48.1 kDa |
UniProt ID | A0A1U8JT65 |
Protein Sequence | MTSDGATSTPAPRRKPSWRERENNRRRERRRRAIAAKIYTGLRAQGNYNLPKHCDNNEVLKALCSEAGWVVEDDGTTYRKGCKPPPIDIGGSSSKITPFSSQNPSPLSSAFPSPIPSCQVSPSSSSYPSPTRFDANNPSTLLPFLRNAIPSSLPPLRISNSAPVTPPLSSPTSRNPKPLPNWETIAKESMASFNYPFYAVSAPASPTHRHFHAPATIPECDESDTSTVESGQWISFQKFAPSTSQVPTSPTFFKLVKHLPPQNLHNDLGVKDKGRGAEFEFESGHLKPWEGERINDIGMDDLELTLGSGKPQC |
Protein Length | Full Length |
Source | E.coli |
Biological Origin | Gossypium hirsutum (Upland cotton) (Gossypium mexicanum) |
Expression Region | 1-313aa(H295N) |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.