GOLGA3 Antibody

• Catalog Number: orb1247435
• Category: Antibodies
• Description: GOLGA3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HSQSRASKEGPGE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GOLGA3
• UniProt ID: Q08378
• NCBI: NP_005886.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GOLGA3, HGNC:4426, GCP170, MEA-2 , Golgi autoantig
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1247435 (0.1 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.