Golden Syrian Hamster IgG (H&L) antibody (Biotin)

• Catalog Number: orb347127
• Category: Antibodies
• Description: Golden Syrian Hamster IgG (H&L) antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Golden Hamster
• Isotype: IgG
• Immunogen: Hamster IgG whole molecule
• Concentration: 1.5 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-Golden Syrian Hamster IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Golden Syrian Hamster IgG (H&L) Antibody, Rabbit-a
• Note: For research use only
• Application notes: Anti-Golden Syrian Hamster IgG Biotin Conjugation has been tested by ELISA and is assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Golden Syrian Hamster IgG.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Purified Rabbit Anti-Golden Syrian Hamster IgG Antibody Biotin Conjugated tested against purified Golden Syrian Hamster IgG Biotin. Each well was coated in duplicate with 1.0 µg of Golden Syrian Hamster IgG. The working dilution is 1:57000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).