Goat IgG (H&L) Secondary Antibody Fluorescein Conjugated

• Catalog Number: orb346985
• Category: Antibodies
• Description: Goat IgG (H&L) antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Goat IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.
• Conjugation: FITC
• Storage: Store anti-goat FITC conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: FITC Conjugated Secondary Antibody, Anti-Goat FITC
• Note: For research use only
• Application notes: Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and western blot. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated. Lane 1: Goat IgG. Load: 50 ng per lane. Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.