Goat IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347031
• Category: Antibodies
• Description: Goat IgG (H&L) antibody (Peroxidase)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:150,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Donkey anti-Goat IgG Antibody Peroxidase conjugati
• Note: For research use only
• Application notes: Anti-Goat IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. It is also suitable in IHC. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Donkey anti-Goat IgG antibody Peroxidase conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n orb346106). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate (p/n orb348651).