Goat IgG (H&L) Antibody

• Catalog Number: orb347027
• Category: Antibodies
• Description: Goat IgG (H&L) antibody
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:23,400-1:43,400, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey anti-Goat IgG Antibody, Donkey anti Goat Ig
• Note: For research use only
• Application notes: Antibody Anti-Goat IgG (H&L) has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG (p/n orb2652767). Lane 2: none. Load: 50 ng per lane. Primary antibody: Goat IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase donkey secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG. Other band(s): none.