Goat IgG (H&L) antibody (FITC)

• Catalog Number: orb347042
• Category: Antibodies
• Description: Goat IgG (H&L) antibody (FITC)
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Goat IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Goat IgG and Goat Serum.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Chicken anti-Goat IgG Antibody fluorescein Conjuga
• Note: For research use only
• Application notes: Anti-Goat IgG Fluorescein Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Chicken anti-Goat IgG Antibody Fluorescein Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Chicken anti-Goat IgG Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT.