GOAT IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb346996
• Category: Antibodies
• Description: GOAT IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Goat IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:13,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum.
• Conjugation: AP
• Storage: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Anti-Goat secondary antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.09% (w/v) Sodium Azide
• Alternative names: rabbit anti-Goat IgG Alkaline Phosphatase Conjugat
• Note: For research use only
• Application notes: Anti-Goat IgG Alkaline Phosphatase conjugate has been tested by ELISA, dot blot, and western blot and is suitable for immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Antigen: Goat IgG Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng Primary antibody: none Secondary antibody: Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Detected using Alkaline Phosphatase Substrate. Block: orb348637 60 min at RT.

ELISA results of purified Rabbit Anti-Goat IgG Alkaline Phosphatase Conjugated Antibody tested against purified Goat IgG. (Green Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer and TMB substrate (p/n orb348651).

Western Blot of Rabbit anti-Goat Antibody Alkaline Phosphatase Conjugated Lane 1: Goat IgG Load: 100 ng per lane Secondary antibody: Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1:1000 for 60 min at RT Block: orb348637 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.