Goat IgG F(c) Rhodamine Antibody

• Catalog Number: orb1463595
• Category: Proteins
• Description: Goat IgG F(c) Rhodamine Antibody
• Tested applications: DOT, SDS-PAGE
• Isotype: IgG F(c)
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain.
• Conjugation: TRITC
• Source: Goat
• Biological Origin: Goat
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat IgG F(c) fragment Rhodamine conjugated, Goat
• Note: For research use only
• Application notes: Goat IgG F(c) fragment Fluorescein conjugated has been tested in dot blot and SDS-Page and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, and utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rhodamine Conjugated Goat IgG Fc. Dotted Rhodamine Conjugated Goat IgG Fc with following concentrations. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng Primary antibody: none Secondary antibody: none Block: orb348637 for 1 HR at RT.

SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.