Goat IgG Fc Antibody

• Catalog Number: orb346981
• Category: Antibodies
• Description: Goat IgG F(c) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Goat
• Isotype: IgG
• Immunogen: Goat IgG F(c) fragment
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab).
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Goat IgG F(c) Antibody, rabbit anti-Go
• Note: For research use only
• Application notes: Anti-Goat IgG F(c) antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Rabbit Anti-Goat IgG F(c) Antibody tested against purified Goat IgG F(c) (purple line), Goat IgG (green line). Each well was coated in duplicate with 1.0 µg. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Dky Anti-Rabbit HRP orb347758.