Goat IgG F(c) Antibody

• Catalog Number: orb346107
• Category: Proteins
• Description: Goat IgG F(c) Antibody
• Tested applications: SDS-PAGE
• Isotype: IgG F(c)
• Concentration: 1.1 mg/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(c). No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain.
• Conjugation: Unconjugated
• Source: Goat
• Biological Origin: Goat
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat IgG F(c) fragment, Goat IgG Fc fragment
• Note: For research use only
• Application notes: Goat IgG F(c) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS PAGE Results of Goat IgG F(c) Fragment. Lane 1: Goat IgG F(c) Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Goat IgG F(c) Non-Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.

SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.