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Catalog Number | orb346106 |
---|---|
Category | Proteins |
Description | Goat IgG Antibody |
Tested applications | ELISA, IHC, SDS-PAGE, WB |
Isotype | IgG |
Concentration | 10 mg/ml |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | Goat IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Goat IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG and anti-Goat Serum. |
Conjugation | Unconjugated |
Source | Goat |
Biological Origin | Goat |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Goat IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Goat immunoglobulin G Read more... |
Note | For research use only |
Application notes | Goat IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment. Lane 3: Reduced Goat IgG F(ab) Fragment. Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE of Goat IgG Whole Molecule. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: 3 µL OPAL Pre-stained Marker. Lane 3: Non-reduced Goat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.
ELISA, IHC | |
Rabbit | |
Goat | |
Polyclonal | |
Biotin |
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