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Catalog Number | orb97644 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TNNI3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 24 |
Target | TNNI3 (aa117-127) |
Entrez | 7137 |
Protein Sequence | KVTKNITEIAD |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TNNI3 antibody, anti troponin I type 3 (cardi Read more... |
Note | For research use only |
Application notes | WB: Approx 28kDa bands observed in Human Heart lysates (calculated MW of 24.0kDa according to NP_000354.4).The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.Recommended concentration: 0.2-0.5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of staining of adult Mouse (A), fetal Mouse (B), adult Rat (C) and adult Pig (D) Heart lysates using Creatine kinase M-type antibody.
Western blot analysis of staining of Human Heart lysate using Creatine kinase M-type antibody.
Immunofluorescence analysis of U2OS cells using TNNI3 antibody
Immunofluorescence analysis of HeLa cells using TNNI3 antibody