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Goat anti Rabbit IgM (Fc specific), conjugated with FITC

Goat anti Rabbit IgM (Fc specific), conjugated with FITC

Catalog Number: orb21386

DispatchUsually dispatched within 5-10 working days
$ 600.00
Catalog Numberorb21386
CategoryAntibodies
DescriptionFluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to Rabbit IgM, Fc specific. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.
Species/HostGoat
ClonalityPolyclonal
Form/AppearanceFITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
ConjugationFITC
Hazard InformationThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
SourcePurified IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
RRIDAB_10938131
StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Buffer/PreservativesFITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
NoteFor research use only
Expiration Date12 months from date of receipt.