Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, ALP conjugated

SKU: orb700364

Description

Goat polyclonal antibody against Goat anti-Rabbit IgG (H&L) conjugated to AP

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution range: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
• Reactivity: Rabbit
• Application Notes:
  This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified Rabbit IgG
• Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
• Purification: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
• Conjugation: ALP

Storage & Handling

• Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Form/Appearance: Liquid
• Disclaimer: For research use only