Goat Anti-Rabbit IgG (H&L) Antibody (biotin)

• Catalog Number: orb1236938
• Category: Antibodies
• Description: Goat Anti-Rabbit IgG (H&L) Antibody (biotin)
• Species/Host: Goat
• Tested applications: ELISA
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Rabbit IgG - whole molecule
• Dilution range: Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Storage: Biotin Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
• Note: For research use only
• Application notes: Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).
• Expiration Date: 12 months from date of receipt.