You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
Goat anti-proteinase 3 / myeloblastin (aa88-98) Antibody
Catalog Number: orb167717
| Catalog Number | orb167717 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to PRTN3. |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, IHC, WB |
| Reactivity | Human |
| Dilution range | ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml |
| Conjugation | Unconjugated |
| MW | 27,8 |
| Target | proteinase 3 / myeloblastin (aa88-98) |
| Entrez | 5657 |
| Protein Sequence | HNVRTQEPTQQ |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | Anti PRTN3 antibody, anti proteinase 3 antibody, a Read more... |
| Note | For research use only |
| Application notes | WB: Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Primary incubation 1 hour at room temperature. Images A and B: Human Bone Marrow and Spleen lysate at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
